Key Features
Specifications
Sample type | gDNA, buccal swabs, FTA card |
Recommended input for gDNA | 1 ng per sample |
Recommended input for FTA card punch | 1.2 mm per sample |
Kit configuration | 96 reaction kit and 384 reaction kit |
Recommended multiplexing capacity | 8 to 96 samples per run |
Locus multiplexing capacity | Simultaneous analysis of 52 genetic markers |
Primer mix content | 27 global autosomal STRs including CODIS and European Standard set 25 Y-STRs including minimum set for the Y Haplotype Reference Database (YHRD) |
Amplicon size | Mean: 235 bp Maximum: 481 bp |
Low-level mixture detection | Detects minor contributors at 5% |
Total library prep time | 7 hours and 15 minutes |
Hands-on library prep time | 1 hour and 30 min |
Total sequencing time | ~28 hours with the MiSeq FGx Reagent Kit ~22 hours with the MiSeq FGx Reagent Micro Kit |